gerM [2018-10-10 14:25:40]

lipoprotein, germination (cortex hydrolysis) and sporulation, required for the assembly of the basal platform of the SpoIIIA-SpoIIQ transenvelope complex during sporulation

locus: BSU28380

pI: 4.68

mw: 39.41 kDa

protein length: 366 aa

gene length: 1098 bp

function: germination (cortex hydrolysis) and sporulation

product: SpoIIQ recruitment factor

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.4|Germination] → [category|SW 4.2.4.2|Additional germination proteins]

Gene

Coordinates: 2,902,002 → 2,903,102

Phenotypes of a mutant

• inactivation of ''[gene|AA519F0D7DC94165C2CF5F7E230502D884898B71|gerM]'' reduces sporulation efficiency to 0.3% that of wild type cells [Pubmed|26735940]

The protein

Catalyzed reaction/ biological activity

• required for the assembly of the basal platform of the [protein|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|SpoIIIAH]-[protein|3282D2C25468776881778006F182FCF322C4821D|SpoIIQ] transenvelope complex during [SW|sporulation] [Pubmed|27381174]
• required for the recruitment of [protein|3282D2C25468776881778006F182FCF322C4821D|SpoIIQ] to the septal membranes on the forespore side [Pubmed|27381174]

Structure

• [PDB|6GZ8] (first GerMN domain) [Pubmed|30266596]
• [PDB|6GZB] (tandem GermN domains) [Pubmed|30266596]

[SW|Localization]

• lipoprotein, outer forespore membrane, localization depends on [protein|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|SpoIIIAH] and [protein|3282D2C25468776881778006F182FCF322C4821D|SpoIIQ] [Pubmed|27381174]

Expression and Regulation

Operons

• genes: [gene|AA519F0D7DC94165C2CF5F7E230502D884898B71|gerM]
  description: [Pubmed|8969504]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|8969504,15699190], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

  regulatory mechanism

  • [protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID]: repression, [Pubmed|15383836], in [regulon|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID regulon]

  regulation

  • expressed early during sporulation in the mother cell ([protein|search|SigE], [SW|SpoIIID]) [Pubmed|8969504,15699190,15383836]

Biological materials

Mutant

• BKE28380 (Δ[gene|AA519F0D7DC94165C2CF5F7E230502D884898B71|gerM]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE28380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACTTTTCCCTCCTAAGG, downstream forward: _UP4_TAAATTGGGATTTTTGATAT
• BKK28380 (Δ[gene|AA519F0D7DC94165C2CF5F7E230502D884898B71|gerM]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK28380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACTTTTCCCTCCTAAGG, downstream forward: _UP4_TAAATTGGGATTTTTGATAT

References

• 7926687,15699190,12107147,8969504,15699190,15383836,18562273,26735940,27381174,30266596